PhD position in Molecular Biology & Biophysics - Hospenthal Group

Research Opportunity in Molecular Biology & Biophysics

The Hospenthal Group at the Institute of Molecular Biology & Biophysics (IMBB) at ETH Zurich is exploring the molecular mechanisms that govern natural transformation in bacteria, a vital mode of horizontal gene transfer. Our group employs a combination of cutting-edge techniques, including cryo-electron microscopy (cryo-EM), biophysical methods, and in vivo functional assays, to investigate the protein machineries involved in this intricate process.

Role Overview

We seek a highly motivated individual to delve into the translocation of DNA from the extracellular environment into the bacterial cytoplasm, facilitating its integration into the genome. Responsibilities will include:

• Expressing and purifying proteins
• Conducting quantitative binding experiments related to protein-protein and protein-DNA interactions
• Producing stable complexes between proteins and/or DNA
• Determining structures of proteins and complexes
• Designing mutants and perturbations for in vivo experiments
• Performing in vivo functional assays, including microbiological approaches and fluorescence microscopy

The position is expected to commence as soon as possible, with an ultimate start date no later than January 2026. Detailed project discussions will occur during the interview process.

Candidate Profile

• MSc Degree in biochemistry, molecular biology, structural biology, or a related field
• A strong motivation to tackle complex mechanistic questions
• Research interests surrounding horizontal gene transfer, natural transformation, membrane proteins, and DNA transporters
• Experience in protein expression and purification
• Familiarity with quantitative biophysical methods (e.g., fluorescence anisotropy, ITC, SPR) is beneficial
• Experience in cryo-EM, including sample/grid preparation, data acquisition, data processing, model building, and interpretation
• Proficiency in English for writing scientific papers and participating in meetings and conferences
• Capability to work independently and contribute to a collaborative lab environment

Work Environment

Become part of a vibrant group dedicated to exploring the molecular mechanisms that underpin bacterial evolution and resistance. This fully-funded, full-time position offers the excellent working conditions synonymous with ETH Zurich, situated on the Hönggerberg campus in Zurich.

• Make a significant impact: Be a part of ETH Zurich, which fosters professional development and contributes positively to society.
• Enjoy numerous benefits, including public transport season tickets, car sharing options, a variety of sports activities through ASVZ, childcare services, and attractive pension benefits.

Commitment to Diversity

ETH Zurich is committed to fostering an inclusive culture that promotes equality of opportunity and values diversity. We nurture a working and learning environment where the rights and dignity of all staff and students are respected.

Join Us

For further information, please refer to our website and learn more about the impact you can make at ETH Zurich, a leader in science and technology dedicated to addressing global challenges.